Circle the correct answer from choices shown in bold or fill inthe blank:
1-When necessary, adjust the intensity of the slideâsillumination using the light source / condenser.The contrast of the image can be adjusted using theiris diaphragm / condenser.Contrast is (briefly explain in one sentence):__________________________________________________________________.
Loss of contrast usually results from too little / toomuch illumination.
2. Never use the 4X / 10X / 40X / 100Xobjective without immersion oil. In this course, will you be usingthis objective? Yes / No.
3- Before removing a slide from the stage, lower /raise the stage and return to the 4X / 10X / 40X /100X objective. When completely finished using themicroscope, leave the light source on / turn off the lightsource.
4. Give the numerical aperture values for the followingobjectives (get these values from the microscope in front of you):4X _______, 10 X _______ and 40 X _______. Numerical aperture meanswhat? __________________________________________.
5-The ability to perceive or distinguish two adjacent points asseparate entities on an image is called ____________. It used as anindicator of lens / specimen quality. An increasein magnification can lead to a reduction in ____________ and____________.
5. What does the term parfocal mean in microscopy? Give a briefdescription.______________________________________________________________________
Circle the correct answer from choices shown in bold or fill inthe blank:
1-When necessary, adjust the intensity of the slideâsillumination using the light source / condenser.The contrast of the image can be adjusted using theiris diaphragm / condenser.Contrast is (briefly explain in one sentence):__________________________________________________________________.
Loss of contrast usually results from too little / toomuch illumination.
2. Never use the 4X / 10X / 40X / 100Xobjective without immersion oil. In this course, will you be usingthis objective? Yes / No.
3- Before removing a slide from the stage, lower /raise the stage and return to the 4X / 10X / 40X /100X objective. When completely finished using themicroscope, leave the light source on / turn off the lightsource.
4. Give the numerical aperture values for the followingobjectives (get these values from the microscope in front of you):4X _______, 10 X _______ and 40 X _______. Numerical aperture meanswhat? __________________________________________.
5-The ability to perceive or distinguish two adjacent points asseparate entities on an image is called ____________. It used as anindicator of lens / specimen quality. An increasein magnification can lead to a reduction in ____________ and____________.
5. What does the term parfocal mean in microscopy? Give a briefdescription.______________________________________________________________________
