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26 Aug 2019
1- Calculate the number of grams needed to make 1 mL of a 1M solution of the oligonucleotide (32nt)
2- You have a 300 μM solution of a primer (45nt) in a volume of 400μL, how many μg of primer do you have total?
3- You received a primer (25nt) which came to you as a powder with a mass of 0.31 mg
a- what concentration of primer do you have when you add 100μL water?
b- How would you make a 5 pmol/μL solution with a total of 100 μL?
PLEASE HELP!
1- Calculate the number of grams needed to make 1 mL of a 1M solution of the oligonucleotide (32nt)
2- You have a 300 μM solution of a primer (45nt) in a volume of 400μL, how many μg of primer do you have total?
3- You received a primer (25nt) which came to you as a powder with a mass of 0.31 mg
a- what concentration of primer do you have when you add 100μL water?
b- How would you make a 5 pmol/μL solution with a total of 100 μL?
PLEASE HELP!
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Irving HeathcoteLv2
27 Aug 2019