Question

A. Indicate whether the following descriptions apply to nutrient, defined, selective,

differential, or enrichment media. If the statement applies to more than one, indicate which ones.

Answers may be used more than once.

____________________ if you want to take an environmental sample and grow a wide variety of

bacteria, you would use this medium

____________________ blood agar is an example, because you can see whether or not microbes

produce substances that lyse red blood cells

____________________ you might make this medium with a specially-formulated salt mixture,

some vitamins, and a refined protein source like peptone

____________________ the lauryl tryptose broth that we used for the presumptive coliform test

is this type of medium- we used it to increase the chance of actually seeing the coliforms

B. Indicate whether the following procedures are best performed with liquid or solid media. If

the statement applies to both, say “both.”

_______________________ you can count colonies of microbes grown with this medium

_______________________ you can grow facultative anaerobes with this medium

_______________________ you can use a spectrophotometer to measure growth in this medium

_______________________ you can prepare this medium by autoclaving it prior to use