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20 May 2018
1. What is the difference in results between a) conducting a cell permeabilization and b) detergent extraction of a living cell to prepare the cytoskeleton?
2. List the components of homogenization buffer, and what each component does.
3. Why is the nucleus removed by a low speed centrifugation?
4. Why does detergent have to be applied well above the critical micelle concentration when detergent extracting a living cell?
5. Would Tris (also called Trizma) buffer be a good buffer to be placed in "intracellular buffer"? Explain your answer.
6. After detergent extraction, how do you concentrate the soluble fraction? Explain this in detail.
7. What happens when you homogenize a membrane such as the rough endoplasmic reticulum?
1. What is the difference in results between a) conducting a cell permeabilization and b) detergent extraction of a living cell to prepare the cytoskeleton?
2. List the components of homogenization buffer, and what each component does.
3. Why is the nucleus removed by a low speed centrifugation?
4. Why does detergent have to be applied well above the critical micelle concentration when detergent extracting a living cell?
5. Would Tris (also called Trizma) buffer be a good buffer to be placed in "intracellular buffer"? Explain your answer.
6. After detergent extraction, how do you concentrate the soluble fraction? Explain this in detail.
7. What happens when you homogenize a membrane such as the rough endoplasmic reticulum?
Hubert KochLv2
21 May 2018
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