Imagine that you are the head of a genome project to analyze the genome of a previously uncharacterized species of a tardigrade. Since this is an imaginary project, you can also imagine that you have as much money as you need, with all of the necessary equipment and qualified and contented colleagues to carry out the analysis. You and members of your lab have recently completed the sequencing of more than 250,000 ESTs or partial transcripts from this species, using many different developmental stages and ages as your source material. You have also been actively sequencing the genome itself so that these ESTs can be located and analyzed, although some of the genome sequence is not yet done.
a. What is a tardigrade and what biological or evolutionary questions might be approached from an analysis of its genome? What arguments would you use to persuade your investors and the grant-awarding agencies that the tardigrade genome project is worth pursuing?
b. What is some of the information you will obtain from genome sequencing that you will not obtain from sequencing ESTs?
c. What is some of the information will you obtain by sequencing ESTs that you will not get from sequencing the genome itself?
d. Describe how you will use the ESTs in combination with the genome sequence to identify protein-coding genes and begin to annotate the genome.
*Genetics- please answer in great detail
Imagine that you are the head of a genome project to analyze the genome of a previously uncharacterized species of a tardigrade. Since this is an imaginary project, you can also imagine that you have as much money as you need, with all of the necessary equipment and qualified and contented colleagues to carry out the analysis. You and members of your lab have recently completed the sequencing of more than 250,000 ESTs or partial transcripts from this species, using many different developmental stages and ages as your source material. You have also been actively sequencing the genome itself so that these ESTs can be located and analyzed, although some of the genome sequence is not yet done.
a. What is a tardigrade and what biological or evolutionary questions might be approached from an analysis of its genome? What arguments would you use to persuade your investors and the grant-awarding agencies that the tardigrade genome project is worth pursuing?
b. What is some of the information you will obtain from genome sequencing that you will not obtain from sequencing ESTs?
c. What is some of the information will you obtain by sequencing ESTs that you will not get from sequencing the genome itself?
d. Describe how you will use the ESTs in combination with the genome sequence to identify protein-coding genes and begin to annotate the genome.
*Genetics- please answer in great detail
