Log in
Biology
1
answer
0
watching
249
views
24 Oct 2018

You are making a knockout mouse, after neomycin (positive) selection you treat the surviving cells with gancyclovir (negative selection) and more cells die. What does cell death after gancyclovir treatment indicate?

1. The targeting vector randomly inserted into the genome

The targeting vector did not insert into the genome

The targeting vector inserted into the genome by homologous recombination

Watch
1
answer
0
watching
249
views

For unlimited access to Homework Help, a Homework+ subscription is required.

Nelly Stracke
Nelly StrackeLv2
27 Oct 2018

Unlock all answers

Get 1 free homework help answer.
Already have an account? Log in
Share

Related textbook solutions

Molecular Cell Biology
8 Edition,
Lodish
ISBN: 9781464183393
Biology: Science for Life with Physiology
6 Edition,
Belk
ISBN: 9780134555430
Biology
2nd Edition, 2013
Openstax
ISBN: 9781947172517
Concepts of Biology
OER Edition,
Openstax
ISBN: 9781938168116
Essentials of Biology
5 Edition,
Mader
ISBN: 9781259660269

Related questions

Which of the following statements about pronuclear injection is accurate?

a. DNA is inserted into any specific cell type whose fate has already been determined, such as skin cells.

b. DNA is inserted into a single celled mouse zygote so that the insertion can be passed to most/all cells of the resulting mouse embryo.

c. DNA that is inserted can integrate randomly anywhere in the genome of the mouse.

d. DNA that is inserted integrates at a specific site in the genome via homologous recombination.

e. DNA that is inserted includes an antibiotic resistance marker to be able to select for the embryonic stem cells (growing in dishes) that have incorporated the new DNA.

f. both A and C

g. both B and C

To generate a gene knockout in mice, which of the following is required?

a. a method for screening to verify insertion into the target gene in the mouse chromosome.

b. regions of homology between the researcher-engineered gene and the target gene in the mouse chromosome.

c. mouse Embryonic stem (ES) cells, to increase the chance that many cells in the host mouse including germline cells (which will form sperm and eggs) will carry the change.

d. all of the above

You follow the scheme outlined in to generate a knockout of the mouse gene Myo6, which is involved in motor coordination and movement. You derive ES cells from WT (Agouti-coated) mice, and disrupt one copy of the Myo6 gene in these ES cells. You then you transplant the ES cells into a blastocyst from a black mouse to generate a chimera. As shown in the last step in the figure, you then cross the chimera to a homozygous black mouse.

Some chimeric animals do not produce offspring carrying the gene replacement, while other chimeric animals with the same gene replacement can easily produce offspring carrying the gene replacement. Why?

a. chimeric animals are unable to produce offspring.

b. the gene replacement prevents correct gamete formation.

c. some chimeras have a germline entirely composed of cells from the blastocyst rather than the modified cells whereas others have a germline entirely composed of modified cells rather than cells from the blastocyst.

When you cross the chimera with the black mouse, amongst 20 F1 progeny obtained, you see some Agouti mice and some Black mice. Which of the following statements about the F1 progeny is true?

a. all the F1 that are Agouti will be homozygous for the myo6 knockout allele.

b. all the F1 that are Agouti will be heterozygous for the myo6 knockout allele.

c. 1/2 of the F1 that are Agouti will be heterozygous for myo6 knockout allele.

d. all the F1 that are Black will be homozygous for the myo6 knockout allele.

Q.In the non-9:3:3:1 ratio dihybrid cross below, there are 3 classes of phenotypes: white, pink, red which of the following is NOT true?

9 A_B_ white

3 A_bb pink

3 aaB_ red

1 aabb red

a.) phenotype is dependent on the genotype of gene B

b.) This is an example of recessive episasis

c.) aa is epistatic to B_, and bb

d.) phenotype is dependent on the genotype of gene A

Q,When choosing a selectable marker, you...

a.) choose a enhancer for your gene of interest

b.) choose a rescue gene to save your gene of interest

c.) choose a resistant gene that will allow for colony screening

d.) choose a gene that encodes for DNA replication

Q.The following steps detail an experiment resulting in transgenic mice with a knockout gene for fur color. Recall that agouti (brown) mice are A/A;M/M and black fur coats are a/a;M/M . List the steps in their correct chronological order.

1.Inject transformed agouti embryotic cells into an a/a;M/M embryo to produce A/A;M/m and a/a;M/M altered embryo

2.Self-cross hemizygous mice to produce homozygous transgenic mice (A/-;M/m)

3.Create a a targeting vector containing tk+ for ganciclovir sensitivity, neoR for neomycin resistance, and the gene encoding fur color.

4.Observe mice with chimeric phenotype and cross them with black a/a;M/M mice to produce transgenic hemizygous progeny (A/a;M/m)

5.Isolate all transformed embryonic stem cells that show homologous recombination and inject them into a embryonic stem cells of an agouti mouse.

a.) 3,5,4,1,2

b.) 1,5,3,4,2

c.) 3,5,1,4,2

d.) 5,3,2,4,2

If dog 1 has is black with alleles (A_B_) , and dog 2 is brown with (aaB_), and dog 3 is yellow with (_ _bb) alleles, what kind of epistasis is this and what color would dog 4 be if he was (AAbb).

1. Recessive epistasis, Yellow

2. Recessive epistasis, Black

3. Dominant epistasis, Yellow

4. Dominant epistasis, Black

A gene interaction in which pair of recessive genes at one locus prevents expression of dominant allele at another locus called

a. incomplete dominance

b. epistatis

c. complete dominance

d. polygenic inheritance

HELP WITH BIO MULTI CHOICE QUESTIONS:

QUESTION 2

Which of the following structures discussed in class is NOT found on all bacteria?
Flagella
Capsid
Fimbria
Plasma membrane


QUESTION 3
You are presented with a strain of bacteria that is resistant to the antibiotic penicillin. What steps would you take to confirm that you have a resistant bacterium?
Perform a Gram stain to determine pink stained Gram-positive bacteria.
Perform a Gram stain to determine purple stained Gram-positive bacteria.
Perform a Gram stain to determine pink stained Gram-negative bacteria.
Perform a Gram stain to determine purple stained Gram-negative bacteria.

QUESTION 4
With respect to bacterial growth into a fresh culture tube containing nutrient broth, at which phase do organisms undergo binary fission the most rapidly?
Lag phase
Stationary
Exponential
death

QUESTION 5
How would antibiotic resistance be passed from one bacterium to another bacterium?
Through the flagella via a plasmid.
Through the sex pilus via chromosomal DNA.
Through the sex pilus via a plasmid.
Through the flagella via chromosomal DNA.

QUESTION 6
When an F plasmid integrates into the host chromosome, the strain is referred to as

Hfr.
F'.
F-.
F+.

QUESTION 7
If you remove the peptidoglycan layer from a Gram-positive cell, it would still stain purple with a Gram stain (T/F).
True
False

QUESTION 8
During infection with a bacteriophage, bacterial DNA is packaged into a capsid and will:

be integrated into the genome of a new bacterium upon ligation.
be integrated into the genome of a new virion upon recombination.
be integrated into the genome of a new bacterium upon recombination.
be integrated into the genome of a new virion upon liagation.

QUESTION 9
Which of the following statements best describes most bacteria?
They are generally harmful.
They are limited to living in a few habitats.
They are very common in the environment.
They are responsible for the “common cold.”


QUESTION 10
Prokaryotes lack some parts found in eukaryotic cells, including which item?
a nuclear membrane
DNA
one or more chromosomes
a plasma membrane
all of the above

QUESTION 11
With respect to gram negative bacteria, what is the order of the bacterial cell structure from outside to inside?
Thin peptidoglycan layer, plasma membrane

thick peptidoglycan layer, plasma membrane

outer membrane, thick peptidoglycan layer, plasma membrane

outer membrane, thin peptidoglycan layer, plasma membrane


QUESTION 12
In transformation, a piece of DNA from a dead host can be taken up by living host

True
False

Related Documents

BIO SCI 97
Final Exam
Study Guide
BIO SCI 97 Study Guide - Final Guide: Ti Plasmid, Organism, Natural Selection
plumwoodchuck347
BIO 1140 Lecture Notes - Dna Replication, Rna Editing, Hydrogen Bond
ceriseshark335

Weekly leaderboard

Home
Homework Help 3,900,000
Biology 460,000
Start filling in the gaps now
Log in