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Compare and contrast ion exchange chromatography with the gel filtration chromatography used to separate proteins in the last lab. Which one separated proteins better? Why not just use that method all the time?

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Deanna Hettinger
Deanna HettingerLv2
22 Jan 2018
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Related questions

When considering anion exchange chromatography, which of the following statements best describes how increasing concentrations of NaCl elute the protein from the column?

Option A: The proteins that are bound to the chromatography resin are exchanged for Cl–.

Option B: The Na+ ions that are bound to the chromatography resin are exchanged for proteins.

Option C: The proteins that are bound to the chromatography resin are exchanged for Na+.

Option D: The Cl– ions that are bound to the chromatography resin are exchanged for proteins.

Given your answer to the question above, which of the following methods would be best suited for a one-step purification of lactate dehydrogenase from a hypothetical mixture of the three proteins?

Option A: anion exchange chromatography using a pH 9 buffer

Option B: anion exchange chromatography using a pH 5 buffer

Option C: anion exchange chromatography using a pH 10.5 buffer

Option D: anion exchange chromatography using a pH 7 buffer

Which of the following combinations of methods would be best suited for the purification of cytochrome c from a hypothetical mixture of the three proteins?

Option A: Gel filtration chromatography to obtain the low molecular weight proteins, followed by cation exchange chromatography at pH 8.

Option B: Cation exchange chromatography at pH 10, followed by elution at high [NaCl], and final purification using gel filtration chromatography.

Option C: Anion exchange chromatography at pH 10, followed by elution at high [NaCl], and final purification using gel filtration chromatography.

Option D: Cation exchange chromatography at pH 7, followed by elution at high [NaCl], and final purification using gel filtration chromatography.

How many amino acids are in the longest form of the human DNA methyltransferase?

Option A: 1511

Option B: 1882

Option C: 1606

Option D 1678

Which of the following statements is NOT correct regarding protein separation techniques?

a) Size-exclusion chromatography works on the same principle as polyacrylamide gel electrophoresis.

b) Specific antibodies are required for immunoblotting, antibody affinity chromatography as well as immunoprecipitation.

c) Two-dimensional gel electrophoresis is conceptually the same as combining gel filtration chromatography and ion exchange chromatography.

d) Western blotting is a useful technique to isolate proteins for us in downstream assays.

Can someone answer the question and provide a short explanation? Thanks!

Which of the following statements is NOT correct regarding protein seperation techniques?

A) Size-exclusion chromatography works on the same principle as polyacrylamide gel electrophoresis

B) Specific antibodies are required for immunoblotting, antibody affinity chromatography as well as immunoprecipitation

C) Two-dimensional gel electrophoresis is conceptually the same as combining gel filtration chromatography and ion exchange chromatography

D) Western blotting is a useful technique to isolate proteins for use in downstream assays

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