Biology 2581B Lecture 7: Lecture 11: generating mutants PPT + NOTES
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We would also like to study different types. Methyl transferred to the o6 of guanine. Since you have a methyl group attached to the oxygen now it can only form 2/3 hydrogen bonds. Now it starts to mis pair with thymine. Want to grow plants, induce mutations, then grow more plants tons. With other organisms it takes so much effort to keep them alive. Assume you have ems treated plants, and you get two mutants that look identical. Do they have change in the same gene causeing the same genotype. Fio if mutation in same gene or nah. Cross them they all look mutant mutation is in the same gene (may not be the same location or thing that happens to the gene) If mutation is in same gene cross them in next generation all phenotypes will be mutation complementation assay. Assume that the two mutations are in different genes.