Biology 2581B Lecture 9: Lecture 9 - Genetic Technologies PPT + NOTES
10 Feb 2016
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Losing restriction site after you re ligate them because part of sequence changes. If you wanna cleave few sites - have a restriction site with more bases. Unless mentioned otherwise under the assumption that it"s a complete digest. Double cut using 2 different enzymes @ same time. Can map the order the fragments are in. 18: cut human dna with ecori, cut vector with same restriction enzyme, now can integrate human fragments into vector, now each vector can contain a different fragment of human dna, close gaps ligate. Ecoli takes up plasmid dna not all cells will have. Transform and selectable marker w/ plasmid will survive. But every vector will have a different insert from genomic library. Can change nucleotide sequence being amplified by making minor changes to the primer. All the mrna"s coding for proteins havea poly a tail. Now can use primer with a whole bunch of ts.
