BIO130H1 Lecture Notes - Lecture 8: Transcription Factor Ii B, Transcription Factor Ii F, Transcription Factor Ii H

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25 Feb 2016

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Eukaryotes: structure of a gene, rna polymerases, transcription of protein-coding genes, rna processing. 433-436; 441-454 (including evolutionary implications: coli promoters. Wont start transcribing until it hits +1 site. Sigma factor recognize the promoter sequences, which stran serve as the template is dependent upon the orientation of the trends. *repressor: work with promoter (dont need it for this class) Rnap binds p, in prokaryote, make a rna that codes for more than one protein. Usually product of a single promoter to coordinate expression. (whether it is transcribe is under the control of single promoter) Translation happening at the same time as transcription. Don"t need to coordinate the number of protein-- efficiency. Eukaryotic transcription is more complicated some steps: 5" cap added as soon as it starts; introns removed. Not polycistronic: one mature rna codes only for one protein. Noncoding rna: eg. the template in telomere synthesis. Each of these rnaps is a multi-subunit.

Related Questions

Match the following:

1	Region which lies upstream of thetranslation start site contains the Shine Dalgarno sequence
2	Sequence added to the 3' end of thepre-mRNA which affects mRNA stability
3	Establishes the reading frame andspecifies the first amino acid of the protein chain
4	DNA sequence that the transcriptionapparatus recognizes and binds to initiate transcription
5	Expressed sequences of DNA that arenot removed during alternative splicing
6	Signals the end of translation andthe protein molecule
7	Group of three nucleotides that codefor an amino acid
8	Site begins the coding region of thegene and is located 25-30 nucleotides downstream of the TATAbox
9	Non-coding sequences of DNA thatintervene within coding regions of a gene
	10 Ribosome binding site ineukaryotes; functions in the initiation of translation and mRNAstability
11	A sequence which lies downstream ofthe coding region of the gene; determines the location of the 3'cleavage site in the pre-mRNA molecule
12	Sequence of nucleotides at the 3' endof the mRNA that is not translated into protein; affects thetranslation and stability of the mRNA molecule

A.	5' untranslated region
B.	AAUAAA consensus sequence
C.	Promoter
D.	Transcription start site
E.	3' untranslated region
F.	Introns
G.	Initiation codon (AUG)
H.	Poly(A) tail
I.	5' cap
J.	Termination codon (UAA, UGA, UAG)
K.	Exons
L.	Sense codon

ivorymacaw91

Please help on these. Thank you!

Question 1

Which of the following is NOT a channel in the RNA polymeraseenzyme?

		RNA exit channel
		DNA entry channel
		DNA exit channel
		Nucleotide exit channel

Question 2

A promoter region upstream of a bacterial gene typically has thesequence TTGACA in the -35 position and TATAAT in the -10 position.If there was a 5 bp insertion at the -40 position, such thateverything downstream was shifted (ie: the "-35 element" is now atthe -30 from the transcription start site), which of the followingwill occur?

		Sigma protein will not bind and so no transcription willoccur.
		RNA polymerase will not bind and so no transcription willoccur.
		Sigma protein will bind to its target sequences and sotranscription will start 5bp downstream from the originaltranscription start site.
		Sigma protein will bind as usual and transcription will startappropriately at the transcription start site.

Question 3

A person has a mutation that prevents TFIIH from being produced.Which of the following outcomes will occur?

		No transcription because TFIID will not be able to bind.
		No transcription because the DNA strand will not be opened.
		Transcription will occur, but it will not be in the correctposition.
		Transcription will occur as usual.

Question 4

There are 4 rRNAs produced to make both subunits of theribosome. How many transcripts are required to produce these 4rRNAs?

		1
		2
		3
		4

Question 5

True or False: the 5' 7m-Gppp cap is attached to the start codonof the mRNA.

		True.
		False, the cap is attached to the 5'-untranslated region.
		False - the cap is attached to the 3'-untranslated region.
		False, the cap is attached to the polyA tail.

Question 6

During the splicing mechanism, which of the following joins twoexons together?

		A 2'OH attacks a 5' phosphate
		A 5' phosphate attacks a 2'OH
		A 3'OH attacks a 5' phosphate
		A 5' phosphate attacks a 3'OH Question 7 After their experiment in Neurospora, Beadle and Tatum concludedthat each single genes code for single enzymes. Do you agree withtheir statement, why or why not?

burgundyhedgehog579

BIO130H1 Lecture Notes - Lecture 8: Transcription Factor Ii B, Transcription Factor Ii F, Transcription Factor Ii H

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