BIO130H1 Lecture Notes - Lecture 10: C-Terminus, Eukaryotic Transcription, Pribnow Box
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BIO130H1 Full Course Notes
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Sequences that the sigma factor when attached to rna polymerase (holoenzyme), it recognizes promoter sequences which gets rna polymerase in the right position for transcription. +1 point, first base going to be transcribed: e(cid:448)e(cid:396)(cid:455)thi(cid:374)g goi(cid:374)g to(cid:449)a(cid:396)ds the 5" e(cid:374)d downstream, e(cid:448)e(cid:396)(cid:455)thi(cid:374)g goi(cid:374)g to(cid:449)a(cid:396)ds the 3" e(cid:374)d upstream, +1 point is the division between downstream and upstream. Usually product of a single promoter to coordinate expression. Operator and repressor protein bind upstream of the coding sequences and help to regulate transcription. Rna polymerase will bind to promoter sequences and transcription initiated at +1 site -> transcribed from all 3 genes. Start with making a primary rna transcript (mrna) -> put 5" (cid:272)ap o(cid:374) rna t(cid:396)a(cid:374)s(cid:272)(cid:396)ipt a(cid:374)d (cid:449)hile t(cid:396)a(cid:374)s(cid:272)(cid:396)i(cid:271)i(cid:374)g, you will cut the transcript and do more processing of mrna such as polydenylation and splicing -> mature. Rna -> eventually exported out of nucleus into the cytoplasm. Prokaryotes have very little processing of mrna.