BIO130H1 Lecture Notes - Lecture 5: Endonuclease, Ap Endonuclease, Dna Glycosylase
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BIO130H1 Full Course Notes
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Lecture 5: issues in dna replication and protein transcription. Lagging: behind leading strands there are okazaki fragments (lagging strands) which make up for any gaps in the leading strand, (cid:1009)" (cid:374)u(cid:272)leases (cid:373)ake all (cid:1009)" e(cid:374)ds sho(cid:396)te(cid:396) (cid:894)applies to pa(cid:396)e(cid:374)tal a(cid:374)d (cid:374)e(cid:449) (cid:1009)" e(cid:374)ds(cid:895) For the protection of ends of dna double strands. If (cid:396)epai(cid:396) p(cid:396)otei(cid:374)s see e(cid:448)e(cid:374) dou(cid:271)le st(cid:396)a(cid:374)ds, the(cid:455) thi(cid:374)k it"s a (cid:271)(cid:396)eak i(cid:374) dna a(cid:374)d (cid:449)ill try to stitch the pieces together. In the majority of eukaryotes, this problem is solved by having repetitive dna sequences at the ends of the chromosomes. Adds to (cid:374)u(cid:272)leotides to: (cid:1007)" e(cid:374)ds of pa(cid:396)e(cid:374)tal st(cid:396)a(cid:374)d te(cid:373)plate. Special dna polymerase comes in with ligase to put down a primer and e(cid:454)te(cid:374)ds (cid:1007)" e(cid:374)d. Result: does(cid:374)"t lose i(cid:374)fo(cid:396)(cid:373)atio(cid:374) a(cid:374)(cid:455)(cid:373)o(cid:396)e e(cid:448)e(cid:374) though (cid:1009)" e(cid:374)d is sho(cid:396)t (cid:271)e(cid:272)ause (cid:1007)" ends will be much longer and then okazaki strands can continue to add on to the sho(cid:396)t (cid:1009)" e(cid:374)d until all information is copied.