GENE20001 Lecture Notes - Lecture 17: Homologous Recombination, Hfr Cell, Genetic Distance
Document Summary
* conjugation - bacteria to bacteria direct transfer (sex)/transfer of genetic material from one parent to the other (parent that has received dna is essentially the zygote) e. g. e. coli. * transduction - phage mediated transfer of genetic material. * transformation - uptake of dna from the environment. * donor (male cell) = f+, recipient (female) cell = f- * f+ have pili on surface which are encoded by an episomal dna (sometimes called a plasmid), which is a circular piece of dna in e. coli called the f factor that is independent to the e. coli chromosomes. So, it replicates independently and divides & partitions into daughter cells independently) * genes on the f factor allow conjugation to occur - the genes on the f factor encode the proteins for pili. Transfer usually happens to completion (entire copy of f factor transferred to f- cell) Lecture 17: f- cell becomes f+ cell, the conjugal tube is broken down/breaks.
