GENE20001 Lecture Notes - Lecture 19: Escherichia Coli, Chromosome, Hfr Cell
Document Summary
Most bacteria will be killed but some will take up dna. Requires dna to be taken up and requires recombination into the bacterial chromosome. Functionally it is like transduction except this doesn"t require a phage. However generally we use plasmids that are autonomous. Gene linkage, gene order and map distances can be determined. Principles are the same as for generalised transduction. Frequency of cotransformation is dependent on distance between genes. Gene order can be determined by examining cotransformation frequencies. Transformation efficiency complicates the mapping as not all cells are competent to accept dna. This technique is very useful for bacteria which have poorly defined conjugation or. This technique is very useful for bacteria which have poorly defined conjugation or transduction system (if we don"t know any good transducing phage) Using transformation, genes which are closely linked on the chromosome can be mapped by determining cotransformation frequencies. Finer detail than conjugation but cannot map distant genes.
