BIO 325 Chapter Notes - Chapter 17: Glyphosate, Erythropoietin, Cell Potency
Document Summary
Transgenic mice are made by injecting foreign dna into a pronucleus of a fertilized egg (pronuclear injection) Transgenic drosophila are made by constructing transgenes via insertion into a p element transposon vector. (p-element transformation) Transgenic plants are made by infecting plants with agrobacterium containing the tumor- inducing plasmid, ti, which was engineered to contain the transgene. (t-dna transfer) All these methods result in transgenes being integrated at random locations in the host genome. Wild-type transgenes can be inserted into an embryo homozygous for a recessive mutant allele. If the normal phenotype is restored, the transgene helps to identify the gene that was mutated. Creating reporter constructs allows easy detection of when and in which tissues a gene is turned on or turned off in eukaryotes. Transgenic organisms can be used to produce medically important human proteins, such as insulin, blood clotting factors, and erythropoietin. Transgenic crop plants could potentially produce ingestible vaccines.
