Biology 1002B Chapter Notes - Chapter Outcomes: Sanger Sequencing, Shotgun Sequencing, Minimal Genome
2 May 2019
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Estimate of number of genes necessary to sustain independent life. Find the organism with the smallest possible genome. What genes are in common in all life. Start knocking the genes out -> causing mutations. Takes samples of entire ecosystem -> break dna into pieces -> sequence it-> assemble genes -> look for different genes activities. Basic mechanism of classical vs. automated sanger sequencing. Uses dideoxynucleotides to interrupt dna synthesis in ways to give series of bands we can read. Takes same basic reaction -> attaches fragments to solid surface -> makes multiple copies. Sequences thousands of fragments at the same times. Works because we have millions of copies of genomes. Take millions of copies of the pieces and break them into random pieces. Computer can reassemble these into one continuous sequence. We make a sequence down to minimal genome (knocking genes out) Strategies for annotation of genome sequence (finding orfs) Genes can be coded in the top and bottom strand.
