Biology 1002B Lecture Notes - Lecture 10: Spliceosome, Start Codon, Crispr
19 Dec 2017
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Current trend in cost of dna sequencing: cost of dna sequencing is dropping coming close to . Structural features of dideoxynucleotides: terminate dna synthesis, they lack 3" oh, once this nucleotide gets included nothing else can be added because no free. 3" end: relatively rare, so when dna polymerase is looking for a for instance, it will most likely find a but it might also find the dideoxy a and this will terminate the sequence. Advantages of pyrosequencing over sanger sequencing: helped drop cost of sequencing because sequencing multiple fragments at once. Orfs in dsdna: open reading frame see above, know it is a gene, compare it to other known genes and see if sequence is similar, see if it makes a protein by seeing if it makes a transcript. Genes with highly conserved sequences as a result of divergence from a common ancestral gene are homologous genes.
