BIO 2133 Chapter Notes -Phoebus Levene, Small Interfering Rna, Hydrogen Bond

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Forward genetics versus reverse genetics: forward genetics: looking at the phenotype (appearance) of the individual and then go looking for the genes that cause it. Genetics as an investigative tool: everything that is done in genetics will be based on this diagram (central dogma, central dogma of genetics: dna is transcribed into mrna, mrna is then translated into a protein (see diagram) If protein changes, it can be detected genes are inherited) Historical perspectives: these people are responsible for telling us what the building blocks for dna are, friedrich meischer: meischer was able to isolate a new molecule known as nuclein from the cell nucleus. He was isolating what he thought was the material that was inherited during fertilization and he found out it was acidic. He had obtained the first purification of dna: levene and chargaff: broke down nucleotides and characterized the 4 bases:

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