BIOL 200 Chapter Notes - Chapter 1: Scanning Electron Microscope, Live Cell Imaging, Immunofluorescence
Document Summary
Topic 1. 1 eukaryotic cells & organelles: critically discuss the classical definitions of cells and organelles. Organelles: any number of organized or specialized structures within a living cell: compare and contrast the major characteristics of bacteria and eukaryotes. Topic 1. 2 microscopy: distinguish between the four major classes of microscopy: brightfield light microscopy, fluorescence light microscopy, transmission electron microscopy (tem), and scanning electron microscopy (sem). Brightfield: analysis of cell structure & function. Fluorescence: usually for localization & tracking of proteins (background usually black) Tem: higher resolution limit so more organelles can be viewed. Sem: usually to view surfaces & requires complex preparations with metal coatings: discuss the major advantages and limitations of each of the four major classes of microscopy. Gfp of protein (shows up in image and rest is dark) Epifluorescence: can be blurry or out of focus. More organelles can be seen (mitochondria, golgi, ribosomes)
