BIOLOGY 3UU3 Chapter Notes - Chapter 12: Corticosterone, Pituitary Gland, Paraformaldehyde
13 Apr 2017
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Reversal of maternal programming of stress responses in offspring through methyl. If genome methylation and demethylation in equilibrium, possible to reverse dna methylation by inducing hypermethylation and/or altering chromatin conformation. (cid:120) dietary methionine converted into sam. (cid:120) (cid:120) Sam proposed to activate dna methylation or inhibit demethylation to cause hypermethylation. Study proposed increasing brain methionine levels to cause hypermethylation and reverse maternal programming of gr expression and hpa stress responses. (cid:120) hypothesis supported; stable effects of maternal care potentially reversible. (cid:120) used long-evans hooded rats. (cid:120) High and low lg-abn = ends of a continuum and not distinct populations. Effects of methionine on methylation pattern of exon i7 promoter (cid:120) adults infused with methionine or saline vehicle; differences mapped using sodium bisulfite. (cid:120) Significant differences in methylation of regions of exon l7 gr promoter, within 5" cpg and 3". Suggests alterations of cytosine methylation in adult brain highly specific.
