BIO 4340 Study Guide - Midterm Guide: Biosafety Cabinet, Personal Protective Equipment, Biosafety Level
Document Summary
Biological safety cabinets: the three types of protection by cabinets and protection- personnel, product, and. Environment: chemical fume hoods- only personnel protection. Class i bsc- only personnel and environment protection, no product protection. Class ii bsc, class iii bsc, isolator- all provide personnel, product, and environment protection: hepa filter- high efficiency particular air filter, minimum efficiency of 99. 97% of removal of 0. 3 micron particles, filters are folded. That"s (cid:449)hat gi(cid:448)es it its effi(cid:272)ie(cid:374)(cid:272)(cid:455: hepa filters do not filter out gasses and vapors, only particulates, methods of hepa filter: interception, inertial impaction, diffusion, electrostatic. Forces: interception- interception, or mechanical separation, is the easiest filtration mechanism to envision. A moving particle is blocked when it encounters a passageway or hole smaller than itself. The larger the particle relative to the hole size, the greater the chance of interception: inertial impaction- inertial impaction, also referred to as impingement, occurs as the air-stream passes through a random network of fibers.
