BIO 208L Study Guide - Midterm Guide: Ampicillin, Red Color, Ecori
LAB 1
1. Using the formula weight of Tris (FW = 121.1), how much (to the nearest 0.01 g) is
needed to prepare 50 mL of a 0.50 M solution? 3.03 g
2. pH = - log [H+]
3. Micropipetters are most accurate when delivering volumes of 50-100% of their
total capacity
a. Use a P200 micropipetter to MOST ACCURATELY dispense a volume of 125 µL
4. Place used glass 10-mL pipets in the pipet wash container located at the back of the
room
5. Character to indicate the start of a formula in Microsoft Excel: =
LAB 2
1. Resolution- Property of light microscopes that relates to the ability to distinguish two
points in the specimen (see two points, instead of one)
2. The total magnification through a compound microscope is equal to the magnification of
the ocular times the magnification of the objective.
3. It is acceptable to focus using the coarse focus knob only when the 4X objective is in
alignment.
4. Purpose of Kohler illumination- provide optimal illumination across the field
5. In light microscopy, an ocular reticle is used to measure the relative size of an object.
6.
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Which of the following is FALSE concerning the state of the microscope when one wishes to
remove a specimen slide after viewing?
2
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The objective with the greatest working distance (4X objective) should be in alignment.
The scanning objective (4X objective) should be in alignment.
The objective with the highest magnification should be in alignment. CORRECT
The mechanical stage should be lowered.
Optical lenses of a high quality microscope are cleaned with lens paper
LAB 3
Which of the following types of microscopy will you NOT use to view specimens this week in lab?
• Brightfield microscopy
• Phase contrast microscopy
• Darkfield microscopy
• Electron microscopy
• Fluorescence microscopy
You are to view all of the following specimens with various types of microscopy EXCEPT:
• Diatoms in a prepared mount
• A wet mount of your red blood cells
• A wet mount of your stained buccal cells
• A wet mount of your unstained buccal cells
• A prepared mount of bovine pulmonary artery endotheial cells
The "slider" of the Olympus CX31 "student" microscope condenser provides the ability to perform
phase contrast microscopy, brightfield/light microscropy, darkfield microscopy- NOT
fluorescence microscopy
The condenser is altered most in converting from brightfield optics to darkfield or phase
contrast optics
Toothpicks used for collection of human cheek cells are considered to be a biohazard and would
be most safely discarded in the Biohazard Waste beaker
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Document Summary
It is acceptable to focus using the coarse focus knob only when the 4x objective is in alignment: purpose of kohler illumination- provide optimal illumination across the field. In light microscopy, an ocular reticle is used to measure the relative size of an object. The objective with the greatest working distance (4x objective) should be in alignment. The scanning objective (4x objective) should be in alignment. The objective with the highest magnification should be in alignment. Optical lenses of a high quality microscope are cleaned with lens paper. Which of the following types of microscopy will you not use to view specimens this week in lab: brightfield microscopy, phase contrast microscopy, darkfield microscopy, electron microscopy, fluorescence microscopy. The slider of the olympus cx31 student microscope condenser provides the ability to perform phase contrast microscopy, brightfield/light microscropy, darkfield microscopy- not fluorescence microscopy. The condenser is altered most in converting from brightfield optics to darkfield or phase contrast optics.
