BIO 208L Study Guide - Midterm Guide: Protist, Elodea, Rhodamine
1. _____fluoresce__________ is the property of some compounds that they absorb light
at some wavelength and shortly thereafter emit light at a slightly longer wavelength.
2. In the lab, the fluorescence microscopes we use are said to be epi-fluorescence
microscopes, because the illumination reaches the specimen through the
___objective_______ lens.
3. _______direct_________ immunofluorescence is the technique in which the
fluorochrome is attached to the antibody directed against the molecule of interest.
4. _____indirect___________ immunofluorescence is the technique in which the
fluorochrome is attached to a "secondary" antibody directed against the "primary"
antibody.
5. The ____emission ______ filter is used to insure that only the light coming from the
excited fluorochrome reaches the observer (or the camera).
6. The ___excitation or barrer_______ filter is used to insure that the only the light
appropriate for excitation of fluorochrome reaches the specimen.
7. The use of antibodies in fluorescence is the technique known as
____immunofluorescence_______________.
8. To visualize the binding of a rabbit primary antibody, one would use a fluorochrome-
conjugated anti-____________.
9. To visualize the binding of a mouse monoclonal antibody, one could use a
fluorochrome-conjugated anti-_____________.
10. To visualize the DNA in cells one can use a fluorescent compound known as
____tritc or rhodamine_____ (two possible answers).
11. In the microscope to be used for fluorescence, the filters necessary to observe each
individual fluorochrome are located in a small box known as a ____fliter cube____
_________.
12. A common red fluorochrome used in biology is ___tritc or propidium
iodide_________.
13. A common green fluorochrome used in biology is ___fitc________.
14. The purpose of the dichroic (or mirror) in the fluorescence microscope is to
____reflect_____ wavelengths of light that excite the fluorochrome.
15. The purpose of the dichroic in the fluorescence microscope is to
_____transmit______ wavelengths of light that are emitted by the fluorochrome.
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