MCDB 423 Study Guide - Midterm Guide: Green Fluorescent Protein, Plasmid, Gc-Content

• School: U of M
• Department: Molecular, Cellular and Developmental Biology
• Course: MCDB 423
• Professor: Aaron Reifler

Document Summary

Exam will cover readings, lectures and handouts, molecular biology 1 and 2, electrophysiology. Research project: introduce a single amino acid mutation within an atp dependentreceptor- Steps of creating mutant receptors: primers design using gene runner software or ncbi orf finder, agilent quikchange. You learned how to design primers using ncbi orf finder, agilent quikchange primer design software, and restriction analyzer . Instead of using the designed primers to introduce the mutation using the quickchange kit (pcr/dpni digest and transformation with xl1b cells), you transformed the pre--made mutant. All the following steps will remain similar to previous terms. A mix of multiple choice, ll in the blank and short answer questions. Site directed mutagenesis (the technique wasn"t not performed in class this year but the topic was covered in a lecture : What is it: site-directed mutagenesis is a technique in molecular biology used to make desirable changes in the nucleotide sequence of a target gene.