MCDB 423 Lecture Notes - Lecture 12: Restriction Enzyme, Agarose Gel Electrophoresis, Cytosol
23 Dec 2020
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Schedule: midterm reminder, lab notebook checkin reminder, molecular bio 3 review, molecular bio 4. Preparing template for in vitro transcription reaction: linearizing plasmid, cleaning up template dna. For this experiment, use all the mutant dna you have left from previous experiments. You should have ~35 ul plasmid left: keep tip boxes closed and make sure samples aren"t exposed to air, always use the nuclease free water if you will be mixing it with dna. If you touch your phone, face, etc with gloved hands, change your gloves. Isolated the plasmid dna using mini prep (molbio2) Mol bio 4,5, 6 : in vitro transcription. What we have done so far: design primers for sdm, transformed bacteria with mutant plasmids (molbio1) 3: conformed mutant plasmids by restriction enzyme digestion (molbio3) Current gaol: express the mutant proteins in xenopus oocytes and study its property using electrophysiology.
