BIOS 220 Study Guide - Midterm Guide: Meiosis, Sigma Factor, Protein Structure
8 Sep 2016
School
Department
Course
Professor
Document Summary
Functional rna- active as rna and never translated into protein. Structure of prokaryotic genes: promotor, transcription starts, 5"utr, protein coding area, 3" utr, transcription stops mrna: 5" lines up with 5"utr on gene, translation starts after 5", ends before 3". Eukaryotic transcription: tfiid binds to tata box through tbp : tfiid is a general transcription factor, tfiid(+tata +tbp),attracts other proteins to form the preinitation complex(rnap ii, tfiii, tfiih, Tfiif: rnap ii starts elongation after ctd phosphorylation. The exons are then put together with 7mgppp being labeled at the 5" end and a(150-200) at the other end in order for mrna to be exported and translated. 5" capping: protects mrna, promotes translation and transport, capping enzymes, done very early. Splicing joins the exons together: introns have gu at 5", ag at 3". Splicosome: the protein unit full of snrnps that splice the gene. snrna- form hydrogen bonds with conserved intron sequences. A branch point- oh- is what causes the cleavage.
