BIOL 1107 Study Guide - Quiz Guide: Monochromator, Galvanometer, Photodetector
Document Summary
Spectrophotometry: analysis of substance based on their ability to absorb specific wavelengths of light. Colored: absorb some wavelengths of white light while transmitting others. Oxygenated blood: bright red in color, hemoglobin molecules are absorbing the blue and green wavelengths while transmitting the red wavelengths. Wavelengths absorbed(nm) correspond to color absorbed: longer wavelengths-red absorbed, shorter wavelengths-violet absorbed. Spectrophotometer: used to detect and measure the amount of light absorption at a specific wavelength: transmittance (%t, absorbance (no units) Absorbance: shift in concentration relative to same color. Blank: background reading: contains everything but substance measuring/ being analyzed, must be used to calibrate the instrument before any samples are measured, each time a wavelength setting is changed, the instrument must be recalibrated (set for. 0 absorbance) with the blank: 0 absorbance = 100% transmittance.
