BIOL 1107 Midterm: Lab Practical I Review Notes
Document Summary
Lab 6: introduction to spectrophotometry: vocabulary, effect of acids on ph - when hcl is added to distilled water, the ph decreases rapidly. When hcl is added to distilled water with a buffer, the ph decreases but not as rapidly: effect of bases on ph -when naoh is added to distilled water, the ph increases rapidly. Content: sample preparation and use of the spectrophotometer, always prepared a blank or reference sample, the relationship between absorbance and transmittance: a=log(1/t, determination of absorption spectra and maximal absorbance, finding and using lambda max for best wavelength. Content: calculated rf values, changing colors of spots, add ninhydrin and heat. Isotonic - solutions of the same non-penetrating solute concentration: tonicity - effects of osmosis on living cells. G: hypertonic - solutions of higher solute concentration, hypotonic - solutions of a lower solute concentration, biuret reagent - contains copper sulfate, potassium hydroxide, and sodium potassium tartrate.
