MCD BIO 165A Study Guide - Midterm Guide: Design Of Experiments, The Sequence, Collagen

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Instructions: make your answers clear and concise, use the back of each sheet to write answers if you run out of room, blue or black ink is preferred. (if you write in pencil, you cannot request a regrade. ) You already know which proteins are present in focal adhesions and that they interact with each other. You"d like to know the structure of focal adhesions in terms of how the proteins that compose a focal adhesion are organized with respect to each other. I would generate fusion proteins between each of the different proteins present in focal adhesions and a fluorescent molecule. I would use super resolution microscopy to determine the location of each of the proteins and their relationship in the focal adhesion. You believe that the specific cells that you are studying, smooth muscle cells from blood vessels, bind to extracellular laminin through a specific integrin on the surface of the smooth muscle cells.