MCD BIO 165A Quiz: Quiz Notes Lecture 5 - 10
29 Nov 2016
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Interacts w/ fibronectin on outside, actin on inside. 18 diff alpha and 8 diff beta subunits. Are receptors, bind to ligands, and transmit signals thru cytosolic domains, connect to cytoskeleton. Bent, cytosolic domains are very close and cannot interact w/ partners. Cytosolic domains are very far apart, can interact. Mn2+ - changes from bent to active conformation. Mediated thru signaling events that alter talin level. N terminal domain of talin is fitting in between the subunits of integrin. Head prohibits alpha subunit from further interaction with beta subunit. Thrombin + par receptors -> signal cascade that makes talin move to membrane (location changes) -> affinity for integrin. Y slide to show converging/diverging effects on monolayer. Sterilize under uv - pumps, tubing, filters, reservoir. Valve system v1 applies pressure alternating to reservoirs a and b. Valve syste v2 controls flow inside your slide. Synchronzied switching of v1 and v2 results in unidirectional flow of you slide flow chamber.
