BIO SCI 99 Study Guide - Midterm Guide: Reverse Genetics, Oligonucleotide, Cloning

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redcockroach754

4 May 2020

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Lecture 13 molecular cloning, cdna library and functional complementation (greyed-out text is meant only to be helpful and is optional: molecular cloning. Pcr, synthetic oligonucleotides, commercial vectors, various cloning methods, For the overexpression of protein to be purified (for in vitro studies). To study the effects of introducing or removing a gene from a cell or organism. To study when and where a particular gene is turned on. For creating genomic libraries that can be used for genomic sequencing. For creating cdna libraries that can be used for measuring transcription and conducting function complementation experiments: cdna library.

Related Questions

1. To isolate a gene, a genetic engineer might need:

2. Cloning vectors used in DNA technology are usually:

3. Genomic libraries usually contain:

4. Complimentary DNA is made with the help of:

5. cDNA:

6. In nucleic acid hybridization bacterial colonies with the gene of interest are identified by:

7. A DNA fingerprint is possible because our DNA contains many stretches of base sequences collectively known as:

8. In an electric field DNA migrates to which pole ___? Why ___?

9. The first stage in creating a genetic map is to:

10. To sequence DNA using the dideoxy chain-termination method, which of the following would not be used?

1.Whati is Genetic Technology?What is bacterial transformation?
What is recombinant DNA technology?What is a vector?What is found in a typical plasmid?How can DNA sequenced use the dideoxy chain termination method? Describe the polymerase chain reaction (PCR). What are some advantages and limitations of this procedure?What is a cDNA? How are cDNAs and DNA microarrays used to study gene expression?Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology,Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA moleculOutline the procedures for cloning a eukaryotic gene in a bacterial plasmid.Describe techniques that allow identification of recombinant cells that have taken up a gene of interest.How can you use the lacZ gene to facilitate cloning ?Distinguish between genomic and cDNA libraries.Define genomics and proteomics.Explain how gel electrophoresis is used to analyze nucleic acids and to distinguish between two alleles of a gene.How does dideoxy sequencing work? What is the significance of using dideoxy nucleotides?What are BACs, YACs, and contigs? What are some practical applications of DNA technology?What are the ways in which organisms can be genetically modified?

burgundylion987

1. You have cloned a human gene along with its promoter and ribosome-binding site in a bacterial cloning vector and then introduced the recombinant plasmid into a bacterial host cell that supports the replication and segregation of the cloning vector. Unfortunately, even though you know the whole human gene sequence is present (by DNA hybridization); you cannot get expression of the gene. Which of the following is NOT a reason why there is no expression?

A. The vector cannot replicate

B. The human promoter is not recognized by the bacterial host cell

C. The ribosome-binding site is not recognized by the bacterial host cell

D. The human gene lacks introns, so it is not transcribed by the host bacterium

2. Restriction endonucleases are:

A. sequence-specific

B. able to recognize any 4-, 6-, or 8-base pair sequence

C. always able to produce single stranded tails

D. only able to cleave bacterial DNA

3. Which of the following is NOT a required characteristic of cloning vectors?

A. Genes for replication of vector DNA and segregation to progeny cells

B. Genes for selection of cells carrying the vector (e.g., antibiotic-resistance)

C. DNA sequence for the introduction of foreign DNA where the integrated DNA does not disable the vector

D. None of the above

4. A poly (T) primer is used to produce cDNA from mammalian mRNA. Which of the following would be used to produce cDNA from bacterial mRNA?

A. A poly (T) primer

B. A random DNA sequence primer

C. A poly (A) primer

D. A poly (GC) primer

BIO SCI 99 Study Guide - Midterm Guide: Reverse Genetics, Oligonucleotide, Cloning

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