BIO 203 Study Guide - Midterm Guide: Cytochrome B6F Complex, Citric Acid Cycle, Pyruvate Dehydrogenase
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1. Choose the TWO correct statements describing the citrate cycle.
ATP is an indirect product of the citric acid cycle | |
Acetyl CoA can increase the pool of citric acid cycle intermediates | |
Fatty acids can increase the pool of citric acid cycle intermediates | |
ADP inhibits α-ketoglutarate dehydrogenase | |
The pool of mitochondrial oxaloacetate can be increased by pyruvate | |
All enzymes of the citric acid cycle are found soluble and free in the mitochondrial matrix |
2.Which molecule in the net reaction of the citrate cycle contributes to the inhibition of pyruvate dehydrogenase?
NADH | |
H+ | |
H2O | |
FAD |
3. How would a high NADH to NAD+ ratio be expected to affect the pyruvate dehydrogenase reaction?
The E1 subunit is phosphorylated by pyruvate dehydrogenase kinase, and the catalytic activity of pyruvate dehydrogenase decreases. | |
The last step of the pyruvate dehydrogenase reaction is blocked, resulting in a decrease in activity. | |
The pyruvate dehydrogenase phosphatase-1 enzyme would increase, resulting in pyruvate dehydrogenase activation at an accelerated rate. | |
The pyruvate dehydrogenase kinase enzyme activity would increase, resulting in an increase in pyruvate dehydrogenase activity. |