CAS NE 525 Study Guide - Midterm Guide: Transfection, Dnm1L, Caspase 8
10 Aug 2018
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Document Summary
Monomeric a-syn gets mutated (a53 not a30) oligomerizes and forms beta sheet protofibrils which include ring like structures that can pierce membranes. Damaged mitochondria releases ros, makes da quinone which binds to a-syn and parkin. N-terminal region contains a53 and a30 mutations, it serves to stabilize alpha helix structure when binding to lipid membranes. Ring like structures mechanism of toxicity is different than fibrils. N-terminal region (not in first 30 amino acids) responsible for ring like formation. A30p does not form rings and neither does partial form of alpha syn. Ring like is involved in in early stages forms before aggregates. So aggregates could be protective because it"s taking away toxic ring structures. Tyrosine hydroxylase is marker for dopamine because its enzyme part of dopamine synthesis pathway. Exogenous: signal to initiate apoptosis found outside of cell (coming from another cell) Membrane receptor to translate signal (death domain receptor) Pro-caspase 8 caspase 8 which is group 3 caspase.
