BIO ENG 111 Study Guide - Midterm Guide: Peptide Synthesis, Mrna Display, Fluorenylmethyloxycarbonyl Protecting Group
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Question 0: fill in your name and student id on each page. (1) Covalently links the translated polypeptide to its corresponding mrna. Promoter allows the sequence to be transcribed to rna. The constant sequence is for amplification by rt-pcr and pcr. Question 3: briefly define the following terms: (a) error-prone pcr (4) A non-error checking polymerase is used during pcr to introduce random mutations into a gene for performing directed evolution (b) fmoc group (4) Base-labile amine protecting group used during solid phase peptide synthesis. Name with last name, first: student id: (c) yeast two-hybrid assay (5) One protein, the bait, is linked to the binding domain of a transcriptional activator. The other protein, the prey, is linked to the transcriptional activator"s activating domain. Physical interaction of the prey and bait brings the two domains in proximity allowing for transcriptional activation and expression of a reporter.
