Biochemistry 3381A Study Guide - Midterm Guide: Sulfonyl, Relative Permittivity, Beta Turn

• School: Western University
• Department: Biochemistry
• Course: Biochemistry 3381A
• Professor: Brown Jason

Document Summary

Specific reactions to know (be able to write out, showing structures) include: peptide bond formation hydrolysis of peptide bonds by proteases oxidation/reduction of thiols/disulfides modifications of thiols by maleimides and iodoacetamide/iodoacetic acid maleimides. Usually carried out at ph above 8 so that thiol is deprotonated and more reactive. Equations to know and apply (these equations will not be provided to you): Formula for calculation of charge on a polypeptide (pks will be provided) Calculation of specific activity of an enzyme preparation. Purification and recovery factors for steps of enzyme purification. Activity yield in percent= total activity final/total activity initial * 100. As you purifiy mass goes down, total activity goes down (some is lost 100 percent recovery unlikely) but specific activity goes up, volume and precent recovery drop too. Regarding other equations presented (e. g. coulomb"s law or the electrophoresis equation), you should understand them in principle (i. e. you should be able to recognize the terms and their significance. )