Biochemistry 2280A Study Guide - Aminoacyl-Trna, Lac Operon, Lac Repressor

• School: Western University
• Department: Biochemistry
• Course: Biochemistry 2280A
• Professor: Christopher Brandl

Document Summary

Humans 25k genes, ~5-10k are expressed in any tissue. Cancer is caused by defects in tumor suppressors and oncogenes (transcription factors) Central dogma: dna =transcription> rna =translation> protein. Rna intermediary allows amplification and can be rapidly degraded which allows genes to be shut off. Gene regulation in a eucaryotic cell: rate of transcription (broken down into initiation, elongation, termination, rate of rna processing (converting a rna into a translatable molecule, includes 5" capping and 3". Polyadenylation, splicing of introns, editing: rate of transport of mrna out of nucleus, rate of mrna degradation, rate of translation (initiation, elongation, termination, protein processing (cleavage, phosphorylation, glycosylation, acetylation, protein degradation. Promoter dna sequence required for transcriptional initiation of a gene. Includes sequences that recognize rna polymerase and recognize any gene specific regulatory factors. Terminator dna sequence required for transcriptional termination. Regulatory proteins transcription factors, key molecules in differential transcription of genes.