Biochemistry 2280A Study Guide - Final Guide: Tata-Binding Protein, Activation Function, Chromatin
11 Oct 2017
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Transcription: dna sequence required for transcriptional initiation: promoter. Base pair rules apply: usi(cid:374)g ntps as su(cid:271)strates, (cid:272)hai(cid:374) elo(cid:374)gatio(cid:374) (cid:271)egi(cid:374)s a(cid:374)d pro(cid:272)eeds i(cid:374) a 5"-3" dire(cid:272)tio(cid:374). Structure of bacterial promoters: recognition sites on dna signal the recruitment of rna polymerase holoenzyme, relative to the start site of transcription that occurs at +1, these are found centred at approximately -35 and -10. 10 and -35 sequences when properly spaced are sufficient to recruit the holoenzyme to the promoter: not all promoters have exactly the same sequences. 2 key reasons why some bacterial promoters are transcribed more than others: strength of the basic promoter elements. Trp operon: genes required for tryptophan biosynthesis in e. coli are transcribed as a single unit from a common promoter, from the single mrna transcript, 5 proteins are translated. It is called an operon and is common in bacteria but not found in eukaryotes: rna polymerase is recruited to the.
