Biochemistry 2280A Study Guide - Final Guide: Galactose, Base Pair, Prokaryote
22 Dec 2016
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Perfect base pairings is always better, mismatch lowers tm. 25000 sequences clones, 5000 both ends, 15000 one side, 5000 both ends. Need clone sequence on both end to sequence the ends. Catalytic rna, recognize rna could be catalyze, rna can have catalyst. 1st enzyme, distinguished them from dna, rna has complex structures, can do multiple things. Cha(cid:396)ged trna, (cid:449)ith aa at (cid:1007)", high e(cid:374)e(cid:396)g(cid:455) (cid:271)o(cid:374)d, (cid:396)ead(cid:455) fo(cid:396) t(cid:396)a(cid:374)slatio(cid:374), a (cid:449)a(cid:455) fo(cid:396) it to k(cid:374)o(cid:449) (cid:449)hat the aa goes on, starts to make aa. Have a field for how they act, translation. Matches first two, same trna will recognize different trna because of wobble, last two of anticodon, first two of codon. Microarrays- for cases you have 2 different cell types, want to know what genes are expressed differently, Isolate dna make cdna, label 1 cell with (cancer cell) red forensic, another with green (normal) 20000 human genes different spots, hybridization of genes.
