BIO370Y5 Study Guide - Midterm Guide: Transposable Element, Point Mutation, Hfr Cell
Document Summary
Chemical mutagens are also effective at creating mutations. Transposable elements is the best answer but what advantage(s) does it have over chemical mutagens. Hint: one of the important that needs to be done is to find the gene you are actually mutagenizing. Find out physically where the transposon is and identify where the gene is or locate it. They can result in insertion into the middle of the gene. Chemical mutagens generate point mutation such as base analogs, intercalate bet dna strands. Composite transposons can have a marker gene which allows to induce a mutation and readily select for it or locate it. One of the pieces of dna they were using to make the recombinant dna molecule was sv40 dna- a virus that can cause cancer in primates.
