BIO314H5 Study Guide - Midterm Guide: Differential Centrifugation, Centrifugal Force, Particle Velocity
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High lab costs: ultracentrifuge: ~100 200k, titanium rotor: ~15 30k, preparative centrifuge (floor): ~40 60k, preparative centrifuge (bench): ~30 50k, technician per hour: ~150 300$ When p > p < l v is positive l v is negative. In an isokinetic gradient, the forces which decrease the sedimentation velocity of molecules (i. e. , the increasing buoyancy and decreasing l) and the increasing rcf which accelerates the p molecules are balanced out, so that the molecules move through the gradient depending mainly on their size. Numerous disulfide bonds make rnases very stable enzymes, so mercaptoethanol is used to reduce these disulfide bonds and aqueous phase containing nucleic acid is mixed with the chloroform that removes phenol residues from solution: chloroform in sevag denatures hydrophobic proteins and removes other components that are not soluble in water, such as lipids, chlorophyll, etc, chloroform is toxic and may enter the body by inhalation, ingestion and absorption through the skin irreversibly denature the proteins.
