BIO206H5 Study Guide - Final Guide: Eukaryotic Initiation Factor 3, Exonuclease, Intron
Document Summary
Cytoplasm (nucleoid region) oric four repeats of 9- bp. Dnaa binding site (ttatccaca) flanked by repeats of 13- bp at- rich region. Dnag (primase), dnab (helicase), dnaps i & iii, clamp loader, sliding clamp, When the dna polymerases reach the termination site or when the two replication forks meet. Table 1: prokaryotic replication, transcription, and translation. Up element = - 60 to - 40. Core rna polymerase enzyme (i. e. no sigma), ntps, Intrinsic gc- rich region forming hairpin followed by string of us. Rho dependent c- rich, g- poor rut site bound by factor, ds termination site (may or may not involve hairpin). Rna polymerase has modest nucleolytic activity that allows excision of most recently added ntp. Formyl or fmet removal, protein folding (2o, 3o, 4o), disulfide bridges, cofactor binding, signal sequence cleavage, phosphoryl- , acetyl- , & glycosylation. 70s ribosome, mrna, aminoacyl- trnas, ef- tu, Binding by rfs & hydrolysis of ester bond b/w trna & polypeptide.
