BPS 3101 Final: BPS3101 Review
16 May 2016
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Document Summary
Crispr/cas9 now being used as a molecular tool for genome editing a programmable tool to site-specifically modify genomes. Can do gene knock out and gene editing. You can alter cas9 also to have other functions other than endonuclease. This is for defense against foreign dna (viruses, horizontal gene transfer) Dna binding domain and activator domain, which rna pol binds to. But if bait and prey interact to bring tf domains close together, then. If tf domains physically separated, no transcription transcription can occur. Hybrid here means fusion or chimeric , not like bp hybridization. So a fusion gene makes a fusion protein: 1. Bait fused to dna binding domain of a yeast transcription factor: 2. Shotgun library of possible prey fused to activation domain of yeast. Will see transcription of reporter gene if there is protein-protein interaction. Take advantage of what information is available in the question. Tell if a feature is expected or unexpected.
