ANSC 4050 Study Guide - Midterm Guide: Zinc Finger Nuclease, Somatic Cell Nuclear Transfer, Zinc Finger
3 Apr 2018
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Slide set 10: list the three major approaches that are currently used for gene editing. The three major approaches for gene editing are zinc finger nucleases (zfns), transcription. Activator-like effector nucleases (talens) and clustered, regularly interspaced short. Zinc fingers are small protein structural motifs that can coordinate zinc ions to help stabilize their folds. They function as interaction molecules that bind dna, rna and proteins, and specific zinc fingers can bind to specific dna. The general process involves incorporating sequences from invading dna between crispr repeat sequences into the bacterial host genome, then transcribing crispr rnas (crrnas) with variable sequences transcribed from invading dna, and part of the crispr repeat. From here each crrna hybridizes with the transactivating crispr rna (tracrrna) to form guide rna, and the guide rna forms a complex with the cas9 nuclease to generate a double stranded break on the invading dna. The 20 nt target sequence on crrna allows the.
