BIO 3102 Study Guide - Alder, Ecotone

a) How many different 8-mer sequences of DNA are there? (Hint: There are 16 possible dinucleotides and 64 possible trinucleotides.) We can quantify the information-carrying capacity of the nucleic acids in the following way. Each position can be one of four bases, corresponding to two bits of information. (22 = 4). Thus a chain of 5100 nucleotides corresponds to 2 * 5100 = 10,200 bits or 1275 bytes.

b) How many bits of information are stored in an 8-mer DNA sequence? In the E.coli genome? In the human genome?

c) A typical computer CD can store up to 700 MB of information. Compare each of the three previous calculated values to the amount of information that can be stored in a computer CD.

i need the answers of part b c and d?????????

Insert a random 9-mer containing at least one of each nucleobase in the following sequence: GCCUGUUGG-(N9)-CCAACAAU (a) Calculate the molecular weight, percent GC content and molar absorptivity for the oligomer you designed. (b) Provide a structural representation of your oligo using one letter nucleotide abbreviations. Show any intramolecular interactions you anticipate. (c) Assume that fluorescein and tetramethylrhodamine were successfully bioconjugated to the 5’ and 3’ ends, respectively. Explain how the addition of a ssDNA oligo with a complementary base sequence to your 9-mer would confirm or refute your proposed structure. Use labelled illustrations to demonstrate this interaction i.e. show the hybrid and provide the sequence of the DNA strand. (d) Suppose the hybrid was briefly subjected to N-cyclohexyl-3-aminopropanesulfonic acid (CAPS). What would this yield? Explain and demonstrate.

Insert a random 9-mer containing at least one of each nucleobase in the following sequence: GCCUGUUGG-(N9)-CCAACAAU

(a) Calculate the molecular weight, percent GC content and molar absorptivity for the oligomer you designed.

(b) Provide a structural representation of your oligo using one letter nucleotide abbreviations. Show any intramolecular interactions you anticipate.

(c) Assume that fluorescein and tetramethylrhodamine were successfully bioconjugated to the 5’ and 3’ ends, respectively. Explain how the addition of a ssDNA oligo with a complementary base sequence to your 9-mer would confirm or refute your proposed structure. Use labelled illustrations to demonstrate this interaction i.e. show the hybrid and provide the sequence of the DNA strand.

(d) Suppose the hybrid was briefly subjected to N-cyclohexyl-3-aminopropanesulfonic acid (CAPS). What would this yield? Explain and demonstrate.

This is all the information that was offered. If there is a uncertainity, please specify