BIO 1140 Quiz: Lab #1 Quiz-4

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amethystgrasshopper167

9 Feb 2018

School

University of Ottawa

Department

Biology

Course

BIO 1140

Professor

Caroline Petit- Turcotte

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Document Summary

Coarse focus knob: permits rapid change in distance between the specimen and the objective thereby allowing right focussing. Fine focus knob: permits small changes in distance between the specimen and the objectives allowing the to focus on the final image of the specimen. Ocular or eyepiece: a magnifying element in the microscope, usually 10x. It is through the ocular or eyepiece that one looks at a specimen. Objectives: the magnifying element that is closest to the specimen. Condenser: system of lenses that concentrates light furnished by the luminator. Condenser height adjustment knob: allows one to focus the concentrated light on a specimen. Aperture iris diaphragm: used to reduce glare from unwanted light by adjusting the angle of the cone of light that comes onto the specimen. Magnification factor = measured size of object = ( actual size object a = on screen size of object a actual size object b = on screen size of object b.

Related Questions

Expalin in detail each question and provide a concise answer.

1.You are assigned a microscope that is equipped with 5X, 10X, 20X, 40X objective lenses. Which one of these objective lenses should be used when you first examine a slide specimen under the microscope? Explain why this objective should be used.

2.How will working distance change as you progress from a low power objective to successively higher power objectives?

3. What causes the inversion of image that you observed through the compound

microscope? Which lens is responsible for this phenomenon?

How does the fieldof view change as you progress from a low power objective to successively higher power objectives?

How does the size of the iris diaphragm opening affect contrast? Consider both light colored samples and dark samples (i.e. a white nylon thread and a black hair).

How does the size of the iris diaphragm opening affect resolution? Would this be affected by the magnification used to observe the sample?

7. How does depth of field change as you progress from a low power objective to

successively higher power objectives?

QUESTION 1

The ocular of a compound microscope has a magnification of 10x and the objective has a magnification of 10x. The total magnification of the microscope is ___---

	a.	10x
	b.	20x
	c.	100x
	d.	1000x

QUESTION 2

The part of the microscope that focuses the light on an object is the ___________.

	a.	Iris Diaphragm
	b.	Coarse Focus
	c.	Condenser
	d.	Body Tube

QUESTION 3

The polarizing filter in a PLM that is located above the objective is called the _________________.

	a.	Polarizer
	b.	Analyzer
	c.	Abbe condenser
	d.	Iris

QUESTION 4

A substance that reacts the same to light polarized in any direction is _________________.

	a.	an isotope
	b.	anisotropic
	c.	isotropic
	d.	divergent

QUESTION 5

The part of the comparison microscope that allows the examiner to view two objects simultaneously is called the _________________.

	a.	comparator
	b.	comparison bridge
	c.	spectroscope
	d.	stage

1 points

QUESTION 6

A stereo microscope can best be described as _____________________.

	a.	Two compound microscopes aligned so that they each see a slightly different part of an object.
	b.	Two compound microscopes aligned with a comparison bridge.
	c.	A compound microscope with two separate stages and one ocular.
	d.	A compound microscope with two eyepieces and a camera mount.

QUESTION 7

In microscopy, resolution is a measure of ________________.

	a.	The ability of the lenses to separate two tiny details that are close together.
	b.	The total magnification power of the microscope.
	c.	The empty magnification power of the microscope.
	d.	The ability of an electron microscope to determine the presence of a large number of elements.

QUESTION 8

Two polarizers (polarizing filters) aligned 90Â° from each other will block out all light.

True

False

QUESTION 9

A simple convex lens creates a magnified real image of an object.

True

False

QUESTION 10

A trinocular microscope has three objective lenses mounted on a turret.

True

False

QUESTION 11

Liquids and gases are usually isotropic.

True

False

QUESTION 12

Match each of the following terms with Its Definition

Compound microscope a. Has a polarizer and an analyzer

Electron microscope b. Permits viewing two objects at once

Stereomicroscope c. Consists of a single convex lens

Comparison microscope d. Long working distance, three-dimensional

Simple hand magnifier e. Basic 2 convex lens microscope

Polarizing microscope f. Can magnify images more than 100,000 times

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BIO 1140 Quiz: Lab #1 Quiz-4

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