BIO 1140 Study Guide - Final Guide: Eyepiece, Objective-C, Cyanobacteria
8 Apr 2017
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Lab #1: introduction to microscopy and observation of. Since parfocal, the focus isn"t totally lost when change objectives. Revolving nose piece: supports various objective lenses and allows for changes of magni cation. Objectives: magnifying element which is closest to the specimen, 4x, 10x, 40x, 100x (don"t use). Knobs on the side allows to move the specimen on. Coarse focus knob: allows rapid change of distance between the specimen and objective = rough focusing (don"t use with 40x) Fine focus knob: small changes in distance between the specimen and the objective = nal focusing. Condenser: system of lenses that concentrates the light from the illuminator. Aperture iris diaphragm: reduces glare from unwanted light by adjusting the angle of the cone of light that comes from the condenser. Prokaryotic and eukaryotic cells: prokaryotic no nuclear membrane and membrane bound organelles. Most genetic material consist of single circular dna molecules.
