BIOL 234 Study Guide - Midterm Guide: Mendelian Inheritance, Centimorgan, Variable Number Tandem Repeat
Document Summary
Molecular markers: all have alleles that produce difference sixed pcr products that can be observed by electrophoresis of agarose gels. To map a gene using molecular markers there must be segregation of different alleles. This means the marker alleles in the mutant plant must be different from the marker alleles in the wildtype to which it is crossed. Sslp: short sequence length polymorphisms, a marker where diff. alleles exist because of indels, show different band sizes on gels. I. e. bc of different alleles of the same gene. If close in banding pattern size than the number of nucleotide that likely differs between them is not that great! Genetic recombination is the generation of new combination of alleles for two or more genes such that combination of alleles different from the parents is observed. Crossing over: process of recombination between homologous chromosomes that occurs in prophase of meiosis 1.
