BIOLOGY 2C03 Lecture Notes - Lecture 6: Zygosity, General Agreement On Tariffs And Trade
16 Oct 2015
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The rf value = map distance: ex. Problem: too few genes are identified to make a map: solution: use another genetic marker = dna markers or nucleotide polymorphisms. Dna marker- piece of dna of known size that comes in identifiable variations (alleles) Molecular markers- loci in the genome whose sequence can be detected via techniques. Fluorescently tagging the proteins or alleles or pcr. Locus refers to region on dna not just a location of a gene. Polymorphisms used to help map, genetic marker. Frequency of polymorphisms: 1/350 bp (vary between species) Classes of polymorphisms: snp, single nucleotide polymorphism, includes digested res (snipsnps, str, short tandem repeat/microsatellite, vntr, variable number tandem repeat/minisatellite. Re cut dsdna at specific sequences (sticky or blunt ends) Gel electrophoresis: different lengths of dn a are placed on a gel and run a help of an electrical current; distance depends on charge and size; dna dye to visualize it under uv light.
