BIOL 112 Study Guide - Final Guide: Oxidative Phosphorylation, Atp Synthase, Intermembrane Space

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23 Dec 2016
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BIOL 112 Full Course Notes
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BIOL 112 Full Course Notes
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Enzyme catalyzes transfer of phosphate from a phosphorylated molecule (=substrate) to adp. Location: euk cytosol or mitochondrial matrix or cytosol for bacteria. Oxphos = atp synthesized by oxidative phosphorylation atp synthase. Most of the atp is made by oxphos. Oxphos uses the reduced high energy intermediates (nadh and fadh2 (e- donors)) from glycolysis, acetyl-coa synthesis step, citric acid cycle to create a h+ gradient (proton gradient) across a membrane. The energy from the electron carriers (e- proton) is used to pump h+ across the membrane. Electrons are delivered by high energy intermediates nadh and fadh2. Nadh is being oxidized to nad (give up 2 electrons to complex 1); same for fadh2 to fad but to complex ii. Energy is captured by electrons passed along the etc in step-down process. This drives the energy needed to pump proton (h+) across the mitochondrial membrane. H+ movement from matrix to intermembrane space requires energy (ex: active transport)

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