BIOL308 Study Guide - Final Guide: S Phase, Chromosome, Telomere
11 Jan 2015
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Biol 308 study questions lectures 10-11: list proteins (enzymes and other factors) involved in the process of dna replication in e. coli. Explain briefly the role of each of these proteins in replication. oric site, or origin of replication contains dnaa boxes and 3 x 13 bp sites that are. Dnaa protein recognizes dnaa boxes, binds and opens 13 bp sites (at-rich) if negative supercoiled, requires atp. Dnab/helicase carried to dnaa by dnac chaperone. Helicase unwinds dna at replication fork, forming positive supercoils ahead of it. Toposiomerase ii/gyrase/dnag converts positive supercoils to negative ones that form in front of the primeosome. Single-stranded binding proteins bind single stranded dna, keeping it from reforming the double helix with helicase) Dna primase adds a ~10 nucleotide rna primer at the primosome (associated. Dna polymerase iii adds dntps downstream of the primer from 5" to 3" end by adding them to the 3" free oh group. Multiple subunits of the dna polymerase iii holoenzyme.
